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HYDROXYETHYL METHACRYLATE SORBENTS HEMA
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The high density of hydroxyl groups on the internal surface provides TESSEK Separon HEMA with hydrophilic properties and excellent derivatization possibilities for the preparation of different chromatographic sorbents. Common activation techniques, e.g., cyanogen bromide, triazine and others, can be used for ligand immobilization on TESSEK Separon HEMA materials. However, all those techniques are rather laborious and health hazards are usually involved. To overcome this step, TESSEK offers ready-for-use, activated affinity supports with a long shelf-life. Two different modifications with different levels of activation are available, the epoxy (E, E-L, E-H) and vinyl sulphone (VS, VSL). Epoxy-activated supports react under suitable conditions with a variety of functional groups, especially with amino-, thio-, carboxy- and hydroxy groups. Vinyl sulphone-activated supports exhibit higher reactivity, particularly for hydroxy groups. The suitable level of activation depends on the type of ligand. For the binding of low molecular weight ligands to high surface density, a high level of activation should be used. For macromolecular ligands, especially where biological activity should be retained, a low activation level is necessary.
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